基因表达
小脑
基因
计算生物学
生物
RNA序列
电池类型
基因组
细胞
神经科学
转录组
遗传学
作者
Samuel G. Rodriques,Robert R. Stickels,Aleksandrina Goeva,Clay Martin,Evan Murray,Charles R. Vanderburg,Joshua D. Welch,Linlin M. Chen,Fei Chen,Evan Z. Macosko
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2019-03-29
卷期号:363 (6434): 1463-1467
被引量:1378
标识
DOI:10.1126/science.aaw1219
摘要
Gene expression at fine scale Mapping gene expression at the single-cell level within tissues remains a technical challenge. Rodriques et al. developed a method called Slide-seq, whereby RNA was spatially resolved from tissue sections by transfer onto a surface covered with DNA-barcoded beads. Applying Slide-seq to regions of a mouse brain revealed spatial gene expression patterns in the Purkinje layer of the cerebellum and axes of variation across Purkinje cell compartments. The authors used this method to dissect the temporal evolution of cell type–specific responses in a mouse model of traumatic brain injury. Science , this issue p. 1463
科研通智能强力驱动
Strongly Powered by AbleSci AI