肿瘤坏死因子α
p38丝裂原活化蛋白激酶
一氧化氮
一氧化氮合酶
槲皮素
炎症
氧化应激
药理学
MAPK/ERK通路
活性氧
化学
促炎细胞因子
激酶
生物
生物化学
抗氧化剂
免疫学
内分泌学
作者
Jun Gui,Juriyati Jalil,Zakiah Jubri,Yusof Kamisah
出处
期刊:Cytotechnology
[Springer Nature]
日期:2019-01-01
卷期号:71 (1): 79-89
被引量:18
标识
DOI:10.1007/s10616-018-0267-8
摘要
Parkia speciosa Hassk is a plant found abundantly in the Southeast Asia region. Its seeds, with or without pods, have been used in traditional medicine locally to treat cardiovascular problems. The pathogenesis of cardiovascular diseases involves inflammation and oxidative stress. Based on this information, we sought to investigate the potential protective effects of Parkia speciosa empty pod extract (PSE) on inflammation in cardiomyocytes exposed to tumor necrosis factor-α (TNF-α). H9c2 cardiomyocytes were divided into four groups; negative control, TNF-α, PSE + TNF-α and quercetin + TNF-α. Groups 3 and 4 were pretreated with PSE ethyl acetate fraction of ethanol extract (500 µg/mL) or quercetin (1000 µM, positive control) for 1 h before inflammatory induction with TNF-α (12 ng/mL) for 24 h. TNF-α increased protein expression of nuclear factor kappa B cell (NFκB) p65, p38 mitogen-activated protein kinase (p38 MAPK), inducible nitric oxide synthase, cyclooxygenase-2 and vascular cell adhesion molecule-1 when compared to the negative control (p < 0.05). It also elevated iNOS activity, nitric oxide and reactive oxygen species levels. These increases were significantly reduced with PSE and quercetin pretreatments. The effects of PSE were comparable to that of quercetin. PSE exhibited anti-inflammatory properties against TNF-α-induced inflammation in H9c2 cardiomyocytes by modulating the NFκB and p38 MAPK pathways.
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