水杨酸
扫描仪
阿司匹林
色谱法
碱性水解
吸光度
RGB颜色模型
化学
准确度和精密度
材料科学
分析化学(期刊)
水解
计算机科学
生物医学工程
数学
人工智能
工程类
统计
生物化学
作者
Rodrigo Sens da Silva,Endler Marcel Borges
标识
DOI:10.1021/acs.jchemed.8b00620
摘要
Here, students determine aspirin (acetylsalicylic acid) mass in pharmaceutical tablets using a colorimetric method. Aspirin, salicylate, and salicylic acid do not absorb visible light. Thus, in alkaline medium, acetylsalicylic acid was hydrolyzed to salicylate; then, it was reacted with an acidic Fe(III) solution, and a violet complex was formed. Quantitative analysis was carried out using absorbance measured at 535 nm (standard method) and digital images obtained with a flatbed scanner (proposed method). Results obtained with both methods were compared using an F-test and a t-test; both methods had shown equivalent accuracy and precision at the 95% confidence level. In addition, one-way ANOVA showed that aspirin masses found by five student groups using both methods are equivalent at the 95% confidence level. In the proposed method, samples were placed in a 96 microwell plate, and RGB values were extracted automatically from all wells in less than 5 min using ImageJ’s plugin “ReadPlate”; data obtained were organized using a spreadsheet to determine aspirin mass in pharmaceutical tablets, recovery, and percent error. Our goal was to design a portable, cost-effective, and user-friendly platform and to develop an experimental methodology that can easily be applied to any research and education laboratory using just a flatbed scanner.
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