An AIE‐Based Dual‐Mode Fluorescent Sensing Platform for Rapid and Visual Detection of Hydrogen Peroxide in Food Samples

ABSTRACT Hydrogen peroxide (H 2 O 2 ) is widely used in food processing for its oxidizing, bleaching, and antimicrobial properties, but improper use may cause residual contamination and potential health risks, making on‐site monitoring of H 2 O 2 residues important for food safety control. Conventional instrumental methods require specialized equipment and laborious procedures, whereas many fluorescent probes suffer from aggregation‐caused quenching (ACQ), background interference, and unstable signal output in complex food matrices. Herein, an aggregation‐induced emission (AIE)‐active fluorescent probe ( TCFISB ) was rationally designed by coupling an AIE luminogen with a boronate ester H 2 O 2 ‐responsive unit. H 2 O 2 ‐triggered oxidative cleavage of the boronate ester restores the donor–acceptor configuration, activates intramolecular charge transfer (ICT), and elicits a fluorescence turn‐on response. The AIE character allows stable emission under aggregated or matrix‐rich conditions, mitigating the limitations of conventional fluorophores. TCFISB enables sensitive and selective detection of H 2 O 2 over 1–300 µM, with a detection limit of 0.47 µM and a response time of 15 min. Although many reported H 2 O 2 fluorescent probes rely on a single fluorescence readout and are used predominantly for biological imaging, food‐oriented probes, particularly dual‐mode systems integrating visual recognition, fluorescence detection, and smartphone‐assisted RGB quantification, remain limited. TCFISB therefore provides a food‐oriented dual‐mode strategy, allowing visual discrimination of H 2 O 2 in food matrices and achieving acceptable recoveries of 88.10%–112.92% in spiked food samples. Fluorescence imaging in living cells and zebrafish further confirms its biocompatibility and responsiveness toward exogenous and endogenous H 2 O 2 . These results support TCFISB as a practical AIE‐based dual‐mode probe for rapid and portable H 2 O 2 monitoring in food safety applications.