表达式(计算机科学)
计算机科学
病毒学
生物
程序设计语言
作者
Cheryl Isaac Murphy,Helen Piwnica‐Worms
标识
DOI:10.1002/0471142301.ns0418s10
摘要
Baculoviruses have emerged as a popular system for overproducing recombinant proteins in eukaryotic cells. This unit gives an overview of the baculovirus expression system, including discussion of the baculovirus life cycle, and post-translational modifications that occur in insect cells. In addition, the steps for overproducing proteins in the baculovirus systems are described along with recommendations for choosing an appropriate baculovirus vector and DNA, and reagents and equipment necessary for implementing the whole overexpression system.
科研通智能强力驱动
Strongly Powered by AbleSci AI