微粒
石墨
水平基因转移
基因转移
粒径
粒子(生态学)
细菌
炭黑
化学
环境化学
材料科学
基因
生物
生态学
生物化学
遗传学
有机化学
物理化学
天然橡胶
系统发育树
作者
Xue-Xia Peng,Jian Zhou,Lan Zhang,Rong Tan,Tianjiao Chen,Danyang Shi,Hai‐Bei Li,Zhongwei Yang,Shuqing Zhou,Min Jin,Jùnwén Lǐ,Dong Yang
摘要
There is growing concern about the transfer of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in airborne particulate matter. In this study, we investigated the effects of various types of carbonaceous particulate matter (CPM) on the transfer of ARGs in vitro. The results showed that CPM promoted the transfer of ARGs, which was related to the concentration and particle size. Compared with the control group, the transfer frequency was 95.5, 74.7, 65.4, 14.7, and 3.8 times higher in G (graphene), CB (carbon black), NGP (nanographite powder), GP1.6 (graphite powder 1.6 micron), and GP45 (graphite powder 45 micron) groups, respectively. Moreover, the transfer frequency gradually increased with the increase in CPM concentration, while there was a negative relationship between the CPM particle size and conjugative transfer frequency. In addition, the results showed that CPM could promote the transfer of ARGs by increasing ROS, as well as activating the SOS response and expression of conjugative transfer-related genes (trbBp, trfAp, korA, kroB, and trbA). These findings are indicative of the potential risk of CPM for the transfer of ARGs in the environment, enriching our understanding of environmental pollution and further raising awareness of environmental protection.
科研通智能强力驱动
Strongly Powered by AbleSci AI