星形胶质细胞
核糖核酸
神经科学
细胞
神经细胞
脑细胞
生物
计算生物学
脑组织
神经元
细胞生物学
基因
遗传学
中枢神经系统
作者
Nan Hu,Tianming Gao,Yihua Chen
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 341-351
标识
DOI:10.1007/978-1-0716-3810-1_29
摘要
Single-cell RNA sequencing (scRNA-seq) has been widely applied in neuroscience research, enabling the investigation of cellular heterogeneity at the transcriptional level, the characterization of rare cell types, and the detailed analysis of the stochastic nature of gene expression. Isolation of single nerve cells in good health, especially from the adult rodent brain, is the most difficult and critical process for scRNA-seq. Here, we describe methods to optimize protease digestion of brain slices, which enable yield of millions of cells in good health from the adult brain.
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