肝细胞
细胞凋亡
化学
细胞内
抗氧化剂
细胞毒性
细胞培养
生物物理学
岩藻黄质
脂质代谢
生物化学
细胞生物学
体外
生物
类胡萝卜素
遗传学
作者
Chunyan Wang,Ying Bai,Wei Yin,Bixiang Qiu,Pengfei Jiang,Xiuping Dong,Honglan Qi
标识
DOI:10.1021/acs.jafc.3c01160
摘要
This study reports an encapsulation system for fucoxanthin (FX) through simple affinity binding with gelatin (GE) and then coating with chitosan oligosaccharides (COS). The effects of FX before and after encapsulation on the human hepatocyte cell line (L02) were investigated. FX-GE and FX-GE-COS nanocomplexes exhibited a spherical shape with diameters of 209 ± 6 to 210 ± 8 nm. FX-GE-COS nanocomplexes were found to perform the best with the highest encapsulation efficiency (EE, 83.88 ± 4.39%), improved FX stability, and enhanced cellular uptake on the nanoscale. The cytotoxicity and cell mitochondrial damage of H2O2 exposure to L02 cells decreased with the increase of free-FX and FX-GE-COS nanocomplexes. FX-GE-COS nanocomplexes' intervention decreased the intracellular ROS and inhibited the apoptosis of L02 cells that was induced by H2O2 exposure in a concentration-dependent manner. Lipidomic analysis revealed that FX-GE-COS nanocomplexes could regulate the lipid metabolism disturbed by H2O2 and protected the mitochondrial function of L02 cells. These results suggested that nanoencapsulation enhanced the antioxidant activity of FX to L02 cells, and the constructed FX-GE-COS nanocomplexes have the potential to be an antioxidant nutritional dietary supplement.
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