间质细胞
脂肪组织
间充质干细胞
胶原酶
细胞生物学
组织工程
骨髓
化学
细胞
生物
病理
免疫学
医学
生物医学工程
癌症研究
生物化学
酶
作者
Justyna M. Buchert,Benedict Lotz,Solvig Diederichs,Wiltrud Richter
出处
期刊:Methods in molecular biology
日期:2022-11-11
卷期号:: 75-85
被引量:1
标识
DOI:10.1007/978-1-0716-2839-3_7
摘要
Adipose-derived stromal cells (ASC) are a promising alternative cell source to chondrocytes as well as to bone marrow-derived mesenchymal stromal cells (BMSC) in cartilage tissue engineering and repair. Here we describe ASC isolation from liposuction by-products by collagenase-based tissue digestion combined with cell filtration and followed by monolayer attachment and expansion culture. Quality control requires confirmation of correct surface marker expression and multilineage differentiation potential by a trilineage differentiation assay.
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