卵清蛋白
免疫系统
食物过敏
免疫学
平衡/c
犬尿氨酸
肠道菌群
双歧杆菌
脂多糖
化学
生物
过敏
色氨酸
乳酸菌
生物化学
氨基酸
发酵
作者
Zhongliang Wang,Jie Zhang,Jian Yuan,Fangfang Min,Jinyan Gao,Wenfeng Liu,Meijia Huang,Jingjing Wu,Hongbing Chen
出处
期刊:Food & Function
[The Royal Society of Chemistry]
日期:2024-01-01
摘要
Food allergy (FA), triggered by specific dietary allergens, has emerged as a substantial global concern for food safety and public health. While studies have elucidated changes in immune cells and cytokines associated with allergen exposure, a comprehensive analysis of the host's metabolic features and the interaction between metabolites and the gut microbiota has not been conducted. In this study, egg allergen ovalbumin (OVA) was administered by the oral route to sensitized BALB/c mice to faithfully replicate key aspects of human FA, including severe allergic diarrhea, mast cell infiltration, and elevated levels of serum IgE, mMCPT-1, and Th2 cell hallmark cytokines (such as IL-4, IL-5, and IL-13). Furthermore, the untargeted and targeted metabolomic analyses indicated that FA in mice precipitated a substantial decrease in the tryptophan metabolites indole-3-acrylic acid (IA) and indole-3-lactic acid (ILA). The integration of shotgun metagenome and metabolome data further unveiled that the dysregulation of indole metabolism is related to a decline in the abundance of beneficial bacteria such as Lactobacillus and Bifidobacterium. Additionally, disruption of the tryptophan indole derivative pathway compromises the maintenance of intestinal mucosal function through the AHR signaling pathway, manifested by decreased expression of Reg3g and IL22. Taken together, this study demonstrated that the anaphylaxis triggered by oral ingestion of food allergens can lead to disruptions in tryptophan metabolism, consequently impairing intestinal immune homeostasis.
科研通智能强力驱动
Strongly Powered by AbleSci AI