In Vivo Imaging Techniques for the Human Scalp: A Systematic Review of the Literature

ABSTRACT Objective Scalp inflammation and alopecia are distressing conditions for which patients regularly present to dermatology. Although some diagnoses can be made clinically, others require biopsy, which carries the risk of pain, infection, bleeding, and scarring. This review examines the existing literature regarding noninvasive in vivo imaging techniques and their evidence and utility in evaluating scalp pathology, with a focus on the diagnostics of hair conditions. Methods A systematic literature search was conducted using the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines without timeframe restrictions. The PubMed and Clarivate (Web of Science) databases were searched using the terms (“imaging” OR “in‐vivo imaging” OR “non‐invasive imaging” OR “non‐invasive in vivo imaging” “imaging,” “in‐vivo imaging) AND (“human scalp disorders” OR “scalp” OR “hair loss” OR “alopecia”). Peer‐reviewed randomized control trials (RCTs), prospective studies, retrospective studies, and case series or reports discussing in vivo imaging of the scalp published before 2022 were selected. Results Forty‐two studies were included and discussed; modalities included laser devices ( n = 27), ultrasound (US) ( n = 13), infrared thermography ( n = 1), skin capacitance imaging (SCI), and ultraviolet light–enhanced visualization (ULEV) ( n = 1). The most common laser devices used were reflectance confocal microscopy (RCM), multiphoton microscopy (MPM), and optical coherence tomography (OCT). US techniques included high‐frequency US (HFUS) and US biomicroscopy (UBM). Conclusion Quality imaging of the scalp in the setting of alopecic, neoplastic, and inflammatory diseases is highly sought after. Many of these noninvasive imaging techniques show promise, each with individual advantages and disadvantages in imaging‐specific conditions. Ultimately, noninvasive imaging techniques may be used to optimize patient management and minimize morbidity associated with scalp biopsies.