发光
重组
阳离子聚合
光化学
激发态
配体(生物化学)
化学
电荷(物理)
荧光
材料科学
光电子学
受体
光学
高分子化学
原子物理学
物理
生物化学
基因
量子力学
作者
Erik Debler,Gunnar F. Kaufmann,Michaël M. Meijler,A. Heine,Jenny M. Mee,Goran Pljevaljčić,Angel J. Di Bilio,Peter G. Schultz,David P. Millar,Kim D. Janda,Ian A. Wilson,Harry B. Gray,Richard A. Lerner
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2008-02-29
卷期号:319 (5867): 1232-1235
被引量:53
标识
DOI:10.1126/science.1153445
摘要
The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.
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