大肠杆菌
RNA聚合酶
酶
DNA
聚合酶
核糖核酸
T7 RNA聚合酶
分子生物学
化学
生物化学
琼脂糖
洗脱
生物
色谱法
基因
噬菌体
作者
K. Praveen Kumar,Dipankar Chatterji
出处
期刊:Journal of Biochemical and Biophysical Methods
[Elsevier]
日期:1988-03-01
卷期号:15 (5): 235-240
被引量:24
标识
DOI:10.1016/0165-022x(88)90010-3
摘要
DNA-dependent RNA polymerase from Escherichia coli was purified further by elution through heparin-Sepharose CL-6B column after the enzyme was obtained, partially purified, using Burgess and Jendrisak's method [(1975)Biochemistry 14, 4634] The total yield of the pure protein was 10 mg from 50 g of E.coli cells. The method was found to be very reproducible and convenient. The enzyme preparation had 60% active molecules and the elongation rate of RNA synthesis by this enzyme was measured to be 11 bases/s over delta D111 T7 DNA.
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