石杉碱甲
星形胶质细胞
体外
活力测定
药理学
去氢骆驼蓬碱
MTT法
生物
化学
细胞生物学
乙酰胆碱酯酶
神经科学
生物化学
酶
中枢神经系统
作者
Lushuang Xie,Cen Jiang,Zhang Wang,Xiaohong Yi,Yuanyuan Gong,Yunhui Chen,Yan Fu
标识
DOI:10.1080/09168451.2016.1222265
摘要
Alzheimer's disease (AD) is the most common cause of dementia. Its pathology often accompanies inflammatory action, and astrocytes play important roles in such procedure. Rela(p65) is one of significant message factors in NF-κB pathway which has been reported high expression in astrocyte treated by Aβ. HupA, an alkaloid isolated from Chinese herb Huperzia serrata, has been widely used to treat AD and observations reflected that it improves memory and cognitive capacity of AD patients. To reveal its molecular mechanisms on p65, we cultured astrocytes, built Aβ-induced AD model, treated astrocytes with HupA at different concentrations, assayed cell viability with MTT, and detected p65 expression by immunohistochemistry and PCR. Our results revealed that treatment with 10 μM Aβ1-42 for 24 h induced a significant increase of NF-κB in astrocytes; HupA significantly down-regulated p65 expression induced by Aβ in astrocytes. This study infers that HupA can regulate NF-κB pathway to treat AD.
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