Heat transfer plays an important role in the day-to-day life of humans in terms of science and technology. There is a vast literature available on the work done on heat transfer devices like heat pipe. In the paper, a multi-turn closed-loop PHP having 2 mm ID and 4 mm OD specifications is used in vertical position to compare its performance for three different working fluids, i.e., methanol, ethyl acetate and deionized water with different filling ratios (60% and 64%). From analyzing previous literature and continuous long experimental work on the designed closedloop Pulsating Heat Pipe (PHP) setup, it is concluded that condenser temperature is the key parameter which affects the temperature difference (between evaporator temperature and condenser temperature) and is greatly responsible for the performance improvement of PHP because it is directly proportional to the heat transfer rate of PHP.