Деубиквитинирующий (DUB) модуль SAGA участвует в Pol III-зависимой транскрипции

SAGA, the multicomponent complex responsible for acetylation histone N-terminal lysine residues, is involved in the transcription activation a wide range eukaryote genes. SAGA contains a protein module, DUB, which is responsible for histone deubiquitination. In this paper we show that the DUB module may be found within cells independently SAGA. In the absence of SAGA, the DUB module may be recruited to the promoters Pol III-transcribed genes, but not to the Pol II-dependent promoters. The DUB module is required to recruit transcription factor Brfl, a subunit the Pol III-recruiting TFIIIB complex, to the promoters Pol III-dependent genes. The DUB-module interacts with Pol III in vivo. The DUB-module is essential for recruiting both TFIIIB complexes and PBP complexes to the promoters Pol III-dependent genes.