With the development of DNA nanotechnology, Janus base nanomaterials are a novel family of non-covalent and DNA-inspired nanostructures. They self-assembled from small-molecule building blocks derived from G^C or A^T DNA base pairs. Scientists have made a lot of progress toward the design, synthesis, engineering and applications of Janus base nanomaterials. Here, we will give a brief review on the development and synthesis of Janus base nanomaterials.