Determination of paeoniflorin, naringin, hesperidin, salvianolic acid B, and tanshinone II_A in Jiegu Pills by HPLC-DAD

Objective To establish a quantification method for simultaneous determination of paeoniflorin, naringin, hesperidin, salvianolic acid B, and tanshinone ⅡA in Jiegu Pills by HPLC-DAD. Methods HPLC simultaneously with changing ultraviolet-visible wavelength was used. Megres C18 column(250 mm × 4.6 mm, 5 μm) was used with acetonitrile- 0.1% phosphoric acid solution as mobile phase in gradient elution mode. The detection wavelengths were set at 230 nm(retention time 0 — 15 min), 280 nm(retention time 15 — 45 min), and 270 nm(retention time 45 — 68 min), respectively. Injection volume was 10 μL at the flow rate of 1.0 m L/min. The column temperature was set at 30 ℃. Results There were good linear relationships between peak areas and contents of five components, such as paeoniflorin, naringin, hesperidin, salvianolic acid B, and tanshinone ⅡA in the ranges of 35.27 — 352.70 ng, 8.625 — 86.250 ng, 53.11 — 531.10 ng, 19.34 — 193.40 ng, and 2.142 — 21.420 ng. The average recoveries of five components were 100.54%, 96.78%, 97.45%, 100.25%, and 99.94% with RSD values of 0.48%, 1.07%, 1.11%, 1.18%, and 1.83%, respectively. Conclusion The established method is convenient, accurate with a satisfactory separation, and high sensitivity. It can be used to determine paeoniflorin, naringin, hesperidin, salvianolic acid B, and tanshinone ⅡA in Jiegu Pills with the same chromatogram condition.