牙本质
成牙本质细胞
牙本质形成
牙乳头
基质(化学分析)
化学
材料科学
解剖
生物物理学
生物医学工程
生物
复合材料
色谱法
医学
作者
Wei-jia Ni,Yucheng Li,Jiang Wu,LongXing Ni
出处
期刊:Chinese journal of stomatology
日期:2010-11-01
卷期号:45 (11): 678-683
标识
DOI:10.3760/cma.j.issn.1002-0098.2010.11.011
摘要
Objective To observe the ability of SD rat dental papillae cells forming dentin-like structure induced by millipore filter combined with transforming growth factor-β1 (TGF-β1). Methods The first passage SD rat dental papillae cells were enzymatically dissociated and centrifuged to obtain a cell mass.The cell mass was seeded on the millipore filter combined with TGF-β1. The complex was incubated for 6 d in vitro or transplanted under the renal capsule for 2 weeks. Then the differentiation of dental papillae cells on the filter and the formation of mineral tissue on the implant were analyzed. Results A layer of polarized columnar cells were observed along the surface of the millipore filter, with cell processes extending into the porous media. Dentin sialoprotein(DSP) and dentin matrix protein-1 ( DMP-1 ) were positive in these cells.After 2 weeks, tubular dentin matrix was deposited on the surface of the aligned cells. Scanning electron microscopy showed that the thickness of newly formed tubular dentin was consistent. DSP and DMP-1 were expressed in columnar cells, tubular matrix and the dental papillae cells adjacent to the filter. Conclusions The millipore filter combined with TGF-β1 could effectively recruit progenitors onto its surface and induce odontoblast differentiation, secrete matrix in a homogenous manner, leading to dentinogenesis.
Key words:
Dental papilla; Micropore filters; Cell differentiation; Dentin regeneration
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