A Molecular Imprinting Electrochemical Sensor for L-Tryptophan Determination

A molecular imprinting electrochemical sensor for the determination of L-tryptophan was developed.The molecular imprinting polymer(MIP) was fabricated by the polymerization reaction of ethyl silicate(TEOS),phenyltrimethoxysilane(PTMOS) and phenyltrimethoxysilane(MTMOS).The presence and removal of the template molecule of L-tryptophan in the MIP film were confirmed by cyclic voltammetry.The sensor showed an excellent selectivity and a high sensitivity for L-tryptophan detection,with a linear range from 1.0×10-9 to 1.0×10-5 mol/L(r=0.9958) and a limit of detection as low as 1.0×10-12 mol/L.The sensor was successfully applied to the determination L-tryptophan in the serum samples and the recoveries were in the range of 80.0%~104%.