Composition and Accumulation of High Molecular Weight Glutenin Subunits in Different Winter Wheat Cultivars

A total of 35 currently popular winter wheat cultivars(lines) from the wheat producing area of the Yellow and Huai Valleys of China were used to identify high molecular weight glutenin subunit(HMW-GS) by SDS-PAGE and investigate the accumulation of HMW-GS in differently filling stage.The results indicated that the earliest HMW-GS gene were expressed on approximate 10 days after anthesis during kernel filling stage in bread wheat surveyed.On 25th day after anthesis,all HMW-GS genes surveyed were expressed in bread wheat while different HMW-GS genes possessed different starting time when expressed.Further analyzing results showed that the expressing amount of almost all HMW-GS genes were slowly increased before 20th days after anthesis.After that,it were quickly increased and reached the peak on approximate 35th day after anthesis but slowly decreased after 35th day and stabilized on 40th day after anthesis.Based on this study,the subunit genes 5 and 10 possessed relatively high expressing amount.This study could provide useful information for further illustrating the mechanism of HMW-GS dynamic accumulation and improvement of wheat quality.