HPLC determination of ginsenoside Rg_1,ginsenoside Re in Jiangtang Ziyabiti tablets

Objective:To establish the content measurement criterion of Jiangtang Ziyabiti tablets.Method:The contents of ginsenoside Rg1 and ginsenoside Re in Jiangtang Ziyabiti tablets were determined HPLC,moreover carry on methodology research.Using Shimadzu VP-ODS(250 mm×4.6 mm,5 μm),acetonitrile-0.05%phosphoric acid gradient elution as mobile phase.The flow rate was set at 1.0 mL·min-1 and the column temperature was set at 25 ℃,the wavelength of ditector was 203 nm.Results:ginsenoside Rg1 and ginsenoside Re be able to very good the separation,but also without interference.The linear ranges of ginsenoside Rg1 and ginsenoside Re were from 0.03730 to 0.1972 mg·mL-1(r = 0.9999)and from 0.03892 to 0.2016 mg·mL-1(r = 0.9998)respectively.The mean recoveries of ginsenoside Rg1 and ginsenoside Re were 97.6%with RSD 2.5%(n = 6)and 96.2%with RSD 2.8%(n = 6).Conclusion:The method is sensitive,accurate and can be used for quality control of Jiangtang Ziyabiti tablets.