The possible role of IL—1 autocrine and paracrine in mesangial cell(MSc)

It was reported that autocrine and paracrine of cytokines play a central role in the pathogenesis of glomeru- lonephritis(GN)and glomerulosclerosis(GS).previous studies demanstrated that IL—1 like factor is not only produced by MSc,but also stimulates MSc proliferation.In order to examine the autocrine and paracrine of IL —1 in MSc in molecular biologic level,we explored the effects of rIL—1 on MSc IL—1 and TGFB mRNA gene expression.Total RNA was extracted from subcultured MSc in presence of absence of rIL-1 in 3,6,12,hours and dot blots.The MSc proiferation was a dose dependent of rIL—1,and IL—1 promoted MSc IL—1 and TGFB mRNA gene expression.Our results support the possible role of IL—1 autocrine and paracine in MSc.