Determination of paeoniflorin in Rupixiao Capsules by HPLC

Objective: To establish a HPLC method for the determination of paeoniflorin in Rupixiao Capsules. Methods: Paeoniflorin was separated on a Hypersil BDS C18 column (4.6 mm×250 mm,5μm)with the use of the acetonitriler-0.1% phosphoric acid(13∶87) as the mobile phase,the flow rate was 1.0 ml/min,the column temperature was 30℃ and the detection wavelength was set at 230 nm. Results: Paeoniflorin was well separated from the other components,the linear range of paeoniflorin was 0.552-5.520 μg (r=0.999 8, n=5),the average recovery was 98.7% with RSD of 0.8%(n=6). Conclusion: The method is simple,acurate and can be used to determine the content of paeoniflorin in Rupixiao Capsules.