Optimization Conditions of Cellulose Enzymatic Hydrolysis

The optimal condition of enzymatic hydrolysis was studied by using straw pulp as a raw material.The influences of enzyme dosage,pH value,hydrolysis temperature,pulp concentration and hydrolysis time were investigated,and enzymatic hydrolysis was optimized by orthogonal experiment.The optimal condition of enzymatic hydrolysis was determined as follow:enzyme dose 27,U,pH 5.5,hydrolysis temperature 50,℃,pulp concentration 2%,hydrolysis time 60,h.Under this condition,the cellulose enzymatic hydrolysis yield can reach 75.8%.