Measurement of Content of Baicalin from Scutellaria baicalensis in Different Habitat by HPLC

Objective:To measure the content of baicalin in root,stem and leave of Scutellaria baicalensis growing in different habitat.Method:The content of baicalin was determined by HPLC.The chromatographic condition included Kromasil ODS(4.6 mm×250 mm,5 μm) and mobile phase consisting of a mixture of methanol-H2O(53:47).Baicalin was detected at 280 nm.The standard curve was linear in the range of 0.124-0.93 μg with correlation coefficient 0.999 9.The average recovery of baicalin was 98.17% with RSD 1.3%(n=6).Result:The average content of baicalin in different habitat root was 6.97%,6.01%,5.49%,3.50% and 4.38% respectively.In different habitat stem,the average content of baicalin was 0.10%,0.08%,0.09%,0.06% and 0.03% respectively.In different habitat leave,the average content of baicalin was 0.27%,0.26%,0.15%,0.14% and 0.09% respectively.Conclusion:The method of distill is simple and stable.The determination method was quick with good reappearance.Produced in the same area,the content of baicalin of S.baicalensis in different habitat and different organ had significant different.