Objective: To investigate factors of the content of hesperidin from Huoxiang Zhengqi solution,and improve transfer rate of hesperidin.Method: Transfer rate of hesperidin in different factors was determined by HPLC.Result: Controlled size of Citrus reticulata under 10 mm,soaked with 70% ethanol at 60 ℃,and concentrated below 65 ℃,transfer rate of hesperidin was highest.Conclusion: This method could effectively improve transfer rate of hesperidin,and ensure the content of hesperidin from finished product.