The flower color of Carthamus tinctorius L.was divided by the PANTONE colour card,the total flavonoids of Carthamus tinctorius L.was determined by UV-visible spectra and hydroxysafflor yellow A(hydroxysafflor yellow A,HSYA) of Carthamus tinctorius L.was determined by high-performance liquid chromatography(HPLC).The results showed that the total flavonoids and hydroxysafflor yellow A existed positive correlation,the flower color of Carthamus tinctorius L.and the hydroxysafflor yellow A had no relevance,and the color difference and total flavonoids had no significant law.