High-resolution melting analysis: applications in molecular tumor testing

High-resolution melting technique shows great potential as a clinical diagnostic tool because of its cost-effectiveness, convenience and closed-tube format which can prevent the risk of cross contamination.HRM analysis has been widely used in molecular diagnostics in the management of cancer by mutation detection, pre-sequence screening, methylation and copy number variations, which involves cancer screening, diagnosis, individual treatment and prognosis.Take advantage of the high detection sensitivity of HRM, the accuracy of mutation screening method could be improved and the test cost could be reduced by combing HRM analysis with sequencing, which helps to develop the molecular diagnostic technique.(Chin J Lab Med, 2017, 40: 80-83) Key words: Neoplasms; Mutation; Methylation; DNA copy number variations; High resolution melting