伪狂犬病
生物
清脆的
Cas9
病毒学
病毒
减毒疫苗
免疫系统
基因
基因组
基因组编辑
毒力
拉伤
遗传学
解剖
作者
Yan-Dong Tang,Ji-Ting Liu,Tong-Yun Wang,Tongqing An,Ming-Xia Sun,Shujie Wang,Qiong-Qiong Fang,Linlin Hou,Zhen Tian,Xuehui Cai
标识
DOI:10.1016/j.virusres.2016.09.004
摘要
Currently, pseudorabies virus (PRV) variant strains are outbreaking in China; these variants belong to genotype II PRV. The traditional Bartha-K61 vaccine has failed to provide complete protection against the emergent variant strains. Therefore, rapid attenuation of current epidemic strains is needed for effective PRV control. In this study, we report a rapid method for editing the PRV genome using the CRISPR-Cas9 system. We developed a triple gE/gI/TK gene-inactivated HeN1 PRV strain, because mice were more susceptible to PRV infection, we then evaluated the attenuation of PRV in the mice and demonstrated that modified PRV was fully attenuated. Furthermore, the attenuated strain also induced immune protection in response to a parental PRV challenge. Overall, we showed that PRVs can be rapidly attenuated using CRISPR-Cas9 technology, which will be critical for PRV control, especially when new variant PRV strains emerge.
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