Nano-C doped Fe-sheathed MgB2 tapes with different doping levels were fabricated using a powder-in-tube method and an in situ reaction. The effects of nano-C doping on the transition critical temperature, microstructure, critical current density, and flux pinning of MgB2 tapes were studied. Compared to the undoped tapes, critical current density J(c) for all the C-doped samples with different doping level was enhanced by more than an order of magnitude in magnetic fields above 10 T. This result indicates that flux pinning was effectively enhanced by the nano-C doping. Highly dispersed nanoparticles; and the substitutions of B by C are proposed to be responsible for the improvement of J(c) -B properties.