溶解循环
巨病毒
生物
单元格排序
病毒
溶解
人口
基因组
病毒学
细胞
细胞生物学
计算生物学
基因
分子生物学
遗传学
社会学
人口学
作者
Dehia Sahmi-Bounsiar,Paulo Victor de Miranda Boratto,Graziele Pereira Oliveira,Jacques Yaacoub Bou Khalil,Bernard La Scola,Julien Andreani
摘要
During the amoeba co-culture process, more than one virus may be isolated in a single well. We previously solved this issue by end point dilution and/or fluorescence activated cell sorting (FACS) applied to the viral population. However, when the viruses in the mixture have similar morphologic properties and one of the viruses multiplies slowly, the presence of two viruses is discovered at the stage of genome assembly and the viruses cannot be separated for further characterization. To solve this problem, we developed a single cell micro-aspiration procedure that allows for separation and cloning of highly similar viruses. In the present work, we present how this alternative strategy allowed us to separate the small viral subpopulations of Clandestinovirus ST1 and Usurpativirus LCD7, giant viruses that grow slowly and do not lead to amoebal lysis compared to the lytic and fast-growing Faustovirus. Purity control was assessed by specific gene amplification and viruses were produced for further characterization.
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