化学
计算生物学
生物分析
DNA甲基化
化学发光
生物化学
基因
色谱法
基因表达
生物
作者
Tiantian Ma,Qiongwen Zhang,Shuying Zhang,Dan Yue,Faming Wang,Yujie Ren,H.-F. Zhang,Yinuo Wang,Yongjun Wu,Lie Liu,Fei Yu
出处
期刊:Talanta
[Elsevier]
日期:2024-06-01
卷期号:273: 125872-125872
标识
DOI:10.1016/j.talanta.2024.125872
摘要
Gene methylation-related enzymes (GMREs) are disfunction and aberrantly expressed in a variety of cancers, such as lung, gastric, and pancreatic cancers and have important implications for human health. Therefore,it is critical for early diagnosis and therapy of tumor to develop strategies that allow rapid and sensitive quantitative and qualitative detection of GMREs. With the development of modern analytical techniques and the application of various biosensors, there are numerous methods have been developed for analysis of GMREs. Therefore, this paper provides a systematic review of the strategies for level and activity assay of various GMREs including methyltransferases and demethylase. The detection methods mainly involve immunohistochemistry, colorimetry, fluorescence, chemiluminescence, electrochemistry, etc. Then, this review also addresses the coordinated role of various detection probes, novel nanomaterials, and signal amplification methods. The aim is to highlight potential challenges in the present field, to expand the analytical application of GMREs detection strategies, and to meet the urgent need for future disease diagnosis and intervention.
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