瘤胃
发酵
微生物
生物
食品科学
基因组
微生物种群生物学
细菌
生物量(生态学)
稻草
反刍动物
生物技术
生物化学
农学
遗传学
基因
作物
作者
Jinsong Liang,Wei Fang,Qingyan Wang,Muhammad Zubair,Guangming Zhang,Wing‐Kin Ma,Yajing Cai,Panyue Zhang
标识
DOI:10.1016/j.biortech.2021.126004
摘要
Anaerobic fermentation of corn straw with rumen microorganisms as inoculum to produce volatile fatty acids (VFAs) is important for biomass valorization. In this study, dynamic variation in bacterial and fungal community composition, carbohydrate-active enzymes (CAZymes) and key functional genes related with VFA production was explored via metagenomic sequencing. Rumen microorganisms efficiently hydrolyzed and acidified corn straw, and VFA concentration reached 8.99 g/L in 72 h. Bacterial and fungal community significantly changed, but the core genera kept stable. Low pH and VFA accumulation were the main factors affecting bacterial and fungal communities. The positive correlations between bacteria were more complex than those between fungi. Most CAZyme abundance significantly decreased after 72 h fermentation, and functional gene abundance participating in VFA generation also decreased. This study provided new insights into dynamic variation of bacteria and fungi during anaerobic ruminal fermentation in vitro, promoting the application of rumen microorganisms in practice.
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