噬菌体
寄主(生物学)
生物
计算生物学
重编程
合成生物学
蛋白质工程
细胞
遗传学
大肠杆菌
基因
生物化学
酶
作者
Matthew Dunne,Nikolai S. Prokhorov,Martin J. Loessner,P.G. Leiman
标识
DOI:10.1016/j.copbio.2021.02.006
摘要
Bacteriophages (phages) use specialized tail machinery to deliver proteins and genetic material into a bacterial cell during infection. Attached at the distal ends of their tails are receptor binding proteins (RBPs) that recognize specific molecules exposed on host bacteria surfaces. Since the therapeutic capacity of naturally occurring phages is often limited by narrow host ranges, there is significant interest in expanding their host range via directed evolution or structure-guided engineering of their RBPs. Here, we describe the design principles of different RBP engineering platforms and draw attention to the mechanisms linking RBP binding and the correct spatial and temporal attachment of the phage to the bacterial surface. A deeper understanding of these mechanisms will directly benefit future engineering of more effective phage-based therapeutics.
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