荧光
心力衰竭
单胺氧化酶
化学
异丙肾上腺素
生物物理学
斑马鱼
活性氧
内科学
生物化学
医学
酶
生物
物理
基因
量子力学
刺激
作者
Xinming Li,Donglei Shi,Yihe Song,Yixiang Xu,Ying Gao,Wei Qiu,Xin Chen,Xiaokang Li,Yunyuan Huang,Yanjun Feng,Baoli Li,Yuan Guo,Jian Li
标识
DOI:10.1016/j.cclet.2021.08.114
摘要
Monoamine oxidase A (MAO-A) is a prominent myocardial source of reactive oxygen species (ROS), and its expression and activity are strongly increased in failing hearts. Therefore, accurate evaluation of MAO-A activity in cardiomyocytes is of great importance for understanding its biological functions and early diagnosing the progression of heart failure. However, so far, there is no report on the fluorescent diagnosis of heart failure by a specific probe for MAO-A. In this work, two far-red emissive fluorescent turn-on probes (KXS-M1 and KXS-M2) for the highly selective and sensitive detection of MAO-A were fabricated. Both probes exhibit good response to MAO-A, one of which, KXS-M2, performs better than the other one in terms of a fluorescence increment and sensitivity. Using the pioneering probe KXS-M2, specific fluorescence imaging of MAO-A in glucose-deprived H9c2 cardiac cells, zebrafish and isoprenaline-induced failing heart tissues was achieved, proving that KXS-M2 can serve as a powerful tool for the diagnosis and treatment of heart failure.
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