苯丙素
生物
MYB公司
牵牛花
转录因子
基因
WRKY蛋白质结构域
结构基因
遗传学
调节基因
基因表达调控
细胞生物学
基因表达
转录组
生物合成
突变体
作者
Ben Spitzer-Rimon,Moran Farhi,Boaz Albo,Alon Cna’ani,Michal Moyal Ben Zvi,Tania Masci,Orit Edelbaum,Yingnian Yu,Elena Shklarman,Marianna Ovadis,Alexander Vainstein
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2012-12-01
卷期号:24 (12): 5089-5105
被引量:106
标识
DOI:10.1105/tpc.112.105247
摘要
Flower scent is a highly dynamic trait, under developmental, spatial, and diurnal regulation. The mechanism governing scent production is only beginning to be unraveled. In petunia (Petunia hybrida), EMISSION OF BENZENOIDS II (EOBII) controls transcription of both the shikimate pathway-regulating MYB factor ODORANT1 (ODO1) and phenylpropanoid scent-related structural genes. A promoter-activation screen identified an R2R3-MYB-like regulatory factor of phenylpropanoid volatile biosynthesis acting downstream of EOBII, designated EOBI. EOBI silencing led to downregulation of ODO1 and numerous structural scent-related genes from both the shikimate and phenylpropanoid pathways. The ability of EOBI to directly activate ODO1, as revealed by electrophoretic mobility shift assay and yeast one-hybrid analysis, place EOBI upstream of ODO1 in regulating substrate availability for volatile biosynthesis. Interestingly, ODO1-silenced transgenic petunia flowers accumulated higher EOBI transcript levels than controls, suggesting a complex feedback loop between these regulatory factors. The accumulation pattern of EOBI transcript relative to EOBII and ODO1, and the effect of up/downregulation of EOBII on transcript levels of EOBI and ODO1, further support these factors' hierarchical relationships. The dependence of scent production on EOBI expression and its direct interaction with both regulatory and structural genes provide evidence for EOBI's wide-ranging involvement in the production of floral volatiles.
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