Poly(styrene-divinylbenzene) microspheres were prepared by seed swelling polymerization in the presence of tung oil methyl esters. First, the core monodisperse polystyrene microspheres were prepared by a dispersion polymerization method. Using polystyrene microspheres as seed, tung oil methyl esters as a functional monomer, divinylbenzene as crosslinker, poly(styrene-divinylbenzene) microspheres were prepared by seed swelling polymerization. Then they were characterized by using FT-IR spectroscopy, TG/DSC and optical microscopy. Results showed that the tung oil methyl esters did participate in the copolymerization of styrene and divinylbenzene.