尿囊素
化学
脱羧
立体化学
催化作用
药物化学
生物化学
作者
G.P.A. Bongaerts,Godfried D. Vogels
标识
DOI:10.1016/0005-2744(79)90115-3
摘要
Uricase (urate:oxygen oxidoreductase, EC 1.7.3.3.) exposes a positional and steric specificity in the enzymic conversion of urate to allantoin. C-2 of urate was recovered as C-2 of allantoin. By the consecutive oxidation and hydrolysis reactions a levorotatory intermediate was formed, presumably (−)-2-oxo-4-hydroxy-4-carbohydroxy-5-ureido-imidazoline. The absorption and optical rotation dispersion spectra of the intermediate were established. In the presence of borate buffer, the intermediate was transformed to (+)-alloxanate. The decay of the former compound depends on general base and acid catalysis. RS-(±)-allantoin was formed by chemical decarboxylation and S-(+)-allantoin by enzymic decarboxylation.
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