细胞毒性
中性红
乳酸脱氢酶
MTT法
氯化镉
分子生物学
毒性
福尔马赞
化学
活力测定
体外
细胞毒性T细胞
镉
生物化学
生物
酶
有机化学
作者
George Fotakis,John A. Timbrell
标识
DOI:10.1016/j.toxlet.2005.07.001
摘要
The aim of this study was to compare four in vitro cytotoxicity assays and determine their ability to detect early cytotoxic events. Two hepatoma cell lines, namely HTC and HepG2 cells, were exposed to cadmium chloride (0–300 μM) for 3, 5 and 8 h. Following exposure to the toxic metal cytotoxicity was determined with the lactate dehydrogenase leakage assay (LDH), a protein assay, the neutral red assay and the methyl tetrazolium (MTT) assay. In HTC cells no toxicity was observed for any incubation period when the LDH leakage, the MTT and the protein assay were employed whereas the neutral red assay revealed early cytotoxicity starting after incubation of HTC cells with CdCl2 for 3 h. In the case of HepG2 cells the MTT assay reveals cytotoxicity due to CdCl2 exposure after 3 h whereas no such effect is seen with the other three assays. Following 5 h exposure of HepG2 cells to CdCl2, toxicity is observed with the MTT assay at lower concentrations compared to the ones required for detection of toxicity with the LDH leakage and the neutral red assay. In conclusion different sensitivity was observed for each assay with the neutral red and the MTT assay being the most sensitive in detecting cytotoxic events compared to the LDH leakage and the protein assay.
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