REVIEW ON UPDATED METHODS OF LC-MS/MS IN CLINICAL DIAGNOSTICS

AbstractLiquid chromatography-tandem mass spectrometry (LC-MS/MS) has become a cornerstone in clinical diagnostics, offering unparalleled sensitivity, specificity, and versatility. This review explores the latest advancements in LC-MS/MS methodologies and their applications in laboratory medicine and patient care. By combining the separation efficiency of liquid chromatography with the analytical precision of tandem mass spectrometry, LC-MS/MS enables accurate detection and quantification of diverse biomarkers, therapeutic drugs, and metabolites in complex biological matrices. Key features such as multiplex testing, broad analyte detection capabilities, and reduced matrix interference make LC-MS/MS an indispensable tool for modern clinical diagnostics. The review highlights the technique's transformative impact on laboratory workflows, its role in improving diagnostic accuracy, and its potential to address emerging challenges in personalized medicine.