Establishment of Human Osteosarcoma Cell Subtitles with Different Potential of Metastasis

Objectives To get the the human osteosarcoma cell sublines with different potential of metastasis. Metheds The different monocell clones were obtained by the limited dilution method from the human osteosarcoma cell line,sosp- 9607. After preliminary screening out by in vitro invision assay,we selected two cell lines as the high metastasis potential cell sublines and three cell lines as the low metastasis potential cell sublines.Then, the cells lines with different poentials of metastasis were screened out by a serial passage in the mice. Results The two sublines with the different metastasis potential were gotten. Conclusions These two subh'nes came from the same mother cell line. The main difference between them lies in the different metastatic phenotype. This study provides an base for the screening of the gene correlated with metastasis and even for the further metastatic mechanism and clinical study of osteosaroma.