Study on extraction and purification technology of C_(21) steroidal glycosides from root of Cynanchum auriculatum Royle ex Wight

Objective To optimize the extraction and purification process of C21 steroidal glycosides from Cynanchum auriculatum. Methods The best extraction technology of C21 steroidal glucosides is decided by single factor and orthogonal experiment. The purification technology is studied by macroporous adsorption resin technology from resin type selection,removing wastes solvent,elution solvent and elution parameters. Results The content of C21 steroidal glycoside is higher when Baishouwu is extracted in 95%alcohol and refluxing and extracting for 2 times,each time 1. 5h. HPD- 100 macroporous adsorption resin is used to purify C21 steroidal glycosides. When the sample is added to the top of column,then eluting by 2BV water,1BV 20% alcohol and 3BV 70%alcohol,70% alcohol fraction is gathered and concentrated with 49% content of C21 steroidal glycoside. Conclusion The extraction and purification technology decided by this study is simple,with a higher content of C21 steroidal glycosides. This study can provide theory evidence for further study of C21 steroidal glucosides.