Sequence Analysis of EBV-BARFO Gene in Nasopharyngeal Carcinoma from Guangdong Area

Objective:There were variations of EBV genome f rom different areas and they may affe ct the biologic function of EBV(such as LMP1).EBV-BARF0was highly detected in th e patients with nasopharyngeal carc inoma (NPC)and its variations have not been rep orted.This study was designed to determine the sequence and variation of EBV-BARF0gene in the patieats with NPC from Guangdong area.Methods :PCR was used to amplify the EBV-BARF0gene in 20patients with NPC and the production s were sequenced on the ABI377.Results:Comparing with standard B95-8,there were four loci with variances i ncluding:160473(G→T),160545(C→T),160701(C→A),160707(G→C)of sequences and 2(Ala→Ser),26(Leu→Phe),78(Arg→Ser),80(Ala→Pro)of amino acid in 20patients with NPC.Conclusion:Since the BARF0gene of EBV is expressed in all nasopharyngeal carcinoma c ell line and biopsies,but it is not expressed or less frequenly in lymphoma tissues and cell lines,the authors firstly reported the variation of EBV-BARF0which compared with B95-8in the patieats with NPC from Guangdong area.These su ggest that the gene may play an important role in the carcinogenesis and development of nasopharyngeal carcinoma .