EFFECT OF 50% ETHANOLIC EXTRACT OF PEGAGAN HERB (Centella asiatica (L.) Urban) ON CELL PROLIFERATION OF LYMPHOCYTES IN Balb/c MALE MICE INDUCED BY HEPATITIS B VACCINE PENGARUH EKSTRAK ETANOLIK 50% HERBA PEGAGAN (Centella asiatica (L.) Urban) TERHADAP PENINGKATAN PROLIFERASI SEL LIMFOSIT MENCIT JANTAN GALUR Balb/c YANG DIINDUKSI VAKSIN HEPATITIS B

Pegagan (Centella asiatica (L.) Urban) has been known as multi effect plant that empirically used in the traditional medicine. This research aims to eva luate the effect of 50%ethanolic extract of pegagan herb against increased lymphocyte pro liferation cells in Balb/c mice that in duced with hepati tis B vaccine, and also to evaluate triterpen g licosida compound (asiaticosida) of 50% ethanolic extrac t of pegagan herb. The test was done by 60 Balb /c male mices that divided into 6 g roups : control group, testing group that was treated wi th pegagan extract dose of 25mg/KgBB, 50 mg/KgBB, 100mg/KgBB, 200mg/KgBB and 400mg/KgBB. All of mices were vaccinated on day 0 and days 15. Furthermore, identification of triterpen glicosida compound was done by KLT method using BAW 4:1:5 as upper eluen. Lymphocyte cells were isolated and the cells pro liferation measured by MTT reduction method. Data was analyzed statistically using 95% confidence level. The result sho wn that 50% of ethanolic extract of pegagan herb did not give the immuno modulatory effect while the identification test shown that 50% of extrac t of pegagan herb has triterpen g licosida compound (asiaticoside).