Chemical constituents in aerial parts of Pulsatilla chinensis

Objective To study the chemical constituents in the aerial parts of Pulsatilla chinensis.Methods The compounds were isolated and purified by silica gel and ODS column chromatographies.Their structures were identified by physical and chemical properties as well as spectral data.Results Nine compounds were isolated from the 95% ethanol extract in the aerial parts of this plant.Their structures were elucidated as L-chicoric acid(Ⅰ),tiliroside(Ⅱ), apigenin 7-O-(3″-p-coumaryl)-glucoside(Ⅲ),4,6,7-trimethoxy-5-methyl coumarin(Ⅳ),4,7-dimethoxy-5-methyl coumarin(Ⅴ),myo-inositol(Ⅵ),shikimic acid(Ⅶ),1,4-amber acid(Ⅷ),and 5-hydroxy-4-oxo-pentanoic acid(Ⅸ),respectively.Conclusion Compound Ⅰ is a new natural product.All compounds are isolated from this plant for the first time.