In paper,The electrochemical behavior of 1,2,3-trimethylbenzene(TMB) on Ti/nanoTiO2-Pt electrode was investigated by cyclic voltammetry and conditions of direct electrosynthesis of 2,3,5-Trimethylbenzoquinone(TMBQ) by electrolysis methods.The results showed that there are electrocatalytic activities of TMB on theTi/nanoTiO2-Pt Electrode,oxidative peak potential Ep is about 1.7 V and reaction irreversible process.And the current efficiency of TMBQ electrosynthesis is 47 % and the total percent conversion of TMB 58.8 % in ionic membrane electrolysis cell.