Extraction of Proteins from Apple Leaves for Two-Dimensional Electrophoresis Analysis

In order to develop an efficient method about protein extraction which is suitable for apple proteomic analysis, four protocols of total protein extraction from apple leaves, which are trichloroacetic acid/acetone precipitation method (TCA), phenol extraction methanol/ammonium acetate precipitation method, Tris-HCl extraction method and modified Tris-HCl extraction method were compared. The results showed that the modified Tris-HCl extraction method was the most suitable method in protein extraction for two-dimensional electrophoresis (2-DE) from apple leaves based on the highest resolution and more informative spots of2-DE gels with no apparent vertical or horizontal streaking.