Development and characterization of a photo-cross-linked functionalized type-I collagen (Oreochromis niloticus) and polyethylene glycol diacrylate hydrogel

Collagen hydrogels have been widely investigated as scaffolds for tissue engineering due to their biocompatibility and capacity to promote cell adhesion. However, insufficient mechanical strength and rapid degradation properties remain the major obstacles for their applications. In the present study, type-I tilapia collagen (TC) was functionalized to form methacrylated tilapia collagen (MATC) by introducing methacrylic acid, developing a photo-cross-linked PEGDA-MATC hydrogel. The mechanical strength of PEGDA-MATC hydrogel could be tuned by adjusting the pH of the precursor solutions, which was decreased with the pH increased. At a pH 5 condition, PEGDA-MATC showed the highest compressive fracture stress (1.31 MPa). Compared to the PEGDA-TC hydrogel, PEGDA-MATC hydrogel exhibited similar swelling behavior to PEGDA-TC hydrogel in PBS solutions, but higher residual mass ratio (PEGDA-MATC, 213.2 ± 2.8%) than PEGDA-TC hydrogel (199.4 ± 3.8%) when cultured with type-I collagenase. PEGDA-MATC hydrogel showed sustained BSA release capacity for 6 days, and the BSA release ratio was significantly (p < 0.05) decreased with increasing concentration of loaded-BSA (68.6% at 4 mg mL−1, 42.2% at 8 mg mL−1). The PEGDA-MATC hydrogel allowed cell adhesion and proliferation in vitro. These results demonstrated that PEGDA-MATC hydrogel might be a potential scaffold for tissue engineering applications.