清脆的
生物
转导(生物物理学)
转基因
骨髓
Cas9
基因组编辑
基因
基因组
计算生物学
免疫系统
引导RNA
遗传学
免疫学
生物化学
出处
期刊:Methods in molecular biology
日期:2019-11-28
卷期号:: 223-230
被引量:3
标识
DOI:10.1007/978-1-0716-0203-4_14
摘要
CRISPR-Cas9 technology allows for rapid, targeted genome editing at nearly any loci with limited off-target effects. Here, we describe a method for using retroviral transduction to deliver single-guide RNA to primary bone marrow-derived macrophages. This protocol allows for high-throughput reverse genetics assays in primary immune cells and is also compatible with retroviral systems for transgene expression.
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